Journal: Frontiers in physiology

Article Title: Gut microbiota depletion minimally affects the daily voluntary wheel running activity and food anticipatory activity in female and male C57BL/6J mice.

doi: 10.3389/fphys.2023.1299474

Figure Lengend Snippet: FIGURE 1 The experimental procedure for microbiota depletion and feeding schedule. The days of beginning of ad libitum feeding (ALI, ALII), timed restricted feeding (TRF), food deprivation (FD) and collection of cecum and feces are indicated with the arrows.

Article Snippet: Extracted fecal DNA samples were shipped to Transnetyx Microbiome (Cordova, TN) for library preparation and whole-genome sequencing.

Techniques:

Journal: Frontiers in physiology

Article Title: Gut microbiota depletion minimally affects the daily voluntary wheel running activity and food anticipatory activity in female and male C57BL/6J mice.

doi: 10.3389/fphys.2023.1299474

Figure Lengend Snippet: FIGURE 5 Relative abundances of microbiota taxa from control and antibiotic-treated mice. Plot representing bacterial load in control (A) and antibiotics- treated (B) mice. Antibiotics depleted majority of gut microbiota. The “Mouse proportion” represents the number of reads that map to the One Codex database mouse genome divided by the total number of reads, while the “Bacterial proportion” represents the number of reads that map to bacteria at the taxonomic rank divided by the total number of reads. The “Other” category includes any reads classified as non-bacterial microorganisms. Taxonomic analysis showing gut microbiota population shifts at Family level. Relative abundance of top 15 families of microbial taxa in control (C), and antibiotics-treated (D) are shown. Lachnospiraceae (indicated as green) was present in all control mice but depleted in antibiotics treated mice. Erysipelotrichaceae was not detected in both control and antibiotics-treated mice. Top 15 orders and genus were shown in Supplementary Figure S5A–D. The number in the brackets represents NCBI taxonomic ID.

Article Snippet: Extracted fecal DNA samples were shipped to Transnetyx Microbiome (Cordova, TN) for library preparation and whole-genome sequencing.

Techniques: Control, Bacteria

Journal: Animal Bioscience

Article Title: Effect of feeding raw potato starch on the composition dynamics of the piglet intestinal microbiome

doi: 10.5713/ab.22.0045

Figure Lengend Snippet: PCoA based on the weighted Unifrac distance showing the 16S rRNA gene-based dissimilarities calculated from the number of ASVs. (A) Beta diversity and bacterial community structure of the intestinal microbiota among CON, RPS5, and RPS10 at the end of the experiment (20 dpf). The center of the cluster of each group was determined based on the distribution of samples, representing the approximate average community composition. (B) Alteration in bacterial community structure during whole experimental period (0, 5, 10, 15, and 20 dpf) within each group. PCoA, principal coordinate analysis; ASV, amplicon sequence variant; RPS, raw potato starch; CON, piglets fed corn/soybean control formula diet; RPS5, piglets fed control formula diet supplemented with 5% RPS; RPS10, piglets fed control formula diet supplemented with 10% RPS; dpf, days post feeding.

Article Snippet: The sequencing libraries were prepared according to the Illumina 16S metagenomic sequencing library protocols to amplify the V3-V4 region.

Techniques: Amplification, Sequencing, Variant Assay, Starch, Control